Broad-Spectrum Antimicrobial Activity of a Natural Peptide, E-Poly-L-lysine

 

Kazuya Yamanaka, Reiko Kato, Hirotaka Furukawa and Masamichi Muto

Chisso Corporation Yokohama Research Center

Yokohama, Kanagawa, Japan

Chisso America Incorporated

Rye, New York, USA

hfurukawa@chissony.com

 

E-Poly-L-lysine (E-PL), produced by Streptomyces albulus, is a naturally occurring homo polymer (25-35 mer) characterized by an amide linkage between the carboxyl and -amino groups of L-lysine. -PL, shows an antibacterial activity, which is generally considered to be a result of the polycationic nature it shares with quaternary ammonium cationic surface-active agents. In addition, this natural compound is well known as a heat stable and effective in a wide range of pH conditions, and has a wide antimicrobial spectrum, including Gram-positive and Gram-negative bacteria, Yeasts and Molds. With a growing demands for a reduction in the widespread use of chemicals, much attention has focused on its safety and efficacy as a natural food preservative. Recently, the U.S. Food and Drug Administration indicated that they had no questions to the manufacture’s conclusions that the compound is generally recognized as safe (GRAS). Given the potential importance of the characterization of -PL as a food preservative, we have investigated its basic properties and applications in actual food applications. This presentation will discuss the antibacterial properties and effective usage in food products.

In 1997, Hirotaka Furukawa joined Chisso Corp in Japan, which was the sole company producing epsilon-polylysine commercially and had worked for development of new manufacturing process and application of polylysine until 2001. In 2001, he transferred to a national laboratory to join a nano-bio technology-related project. Now very unique nano magnetic beads which were developed through this project have been produced by an affiliate company of Chisso Corp. In 2003, after the development of nano beads, he transferred to Chisso America to obtain the approval of polylysine as food ingredient from the FDA and start sales promotion and marketing of polylysine in the USA.  In 2004, polylysine was concluded as GRAS and now the sale has been strongly promoted.